To elucidate the effects of miR-485-5p/NQO1 interplay on metabolic and physiological functions in CRC cells, experiments were conducted in which miR-485-5p mimics or inhibitors, along with vectors for NQO1 modulation, were introduced into the HCT-116 and DLD-1 cell lines. The gene discussed is NQO1; the disease is colorectal carcinoma.