Pathological classification of hematoxylin and eosin (H&E)-stained spatial regions (Figures S8A, S8B) reveals that COL10A1 is most highly expressed in stromal, lymphocytic, and invasive carcinoma regions, and rarely expressed in adjacent ductal carcinoma in situ, adipose tissue, or normal breast tissue regions (Figure S8C). Here, COL10A1 is linked to invasive carcinoma.