To confirm the effects of ST6GAL1-induced sialylation on CRC cells, we treated SW48-OE and Caco2-EV cells with sialidase (NA), which significantly decreased sialylation in both cell lines, as evidenced by reduced SNA lectin and IFC staining (Figure 6A and 6B, and Figure S6A). This evidence concerns the gene ST6GAL1 and colorectal carcinoma.