On this basis, we combined PA to simulate the high fatty acid environment and established the cell models of the LGHF group and HGHF group in order to further investigate the expression of TNFSF10, SERPINB2, and TNFRSF1A in MAFLD and T2DM-related MAFLD. The gene discussed is TNFSF10; the disease is type 2 diabetes mellitus.