Our current data indicated inactivation of Fxr protected against HFD-FMT administration induced GDM development and had an improved effect on glucose metabolism as reflected by the above reaction only performed in WT mice but not in whole-body Fxr-deficient mice, which suggested that Fxr pathway was involved in the response of HFD-associated gut microbiota disordered BAs metabolites induced GDM development. The gene discussed is NR1H4; the disease is gestational diabetes.