Furthermore, colesevelam intervention alleviated HFD-associated GDM development, improved BAs metabolism, suppressed Fxr, Shp, and Fgf15 activity only in WT mice but not in the Fxr−/− HFD + Colesevelam group and Fxr−/− HFD group. This evidence concerns the gene NR1H4 and gestational diabetes.