Moreover, the proportions of poly-functional effector NK cells, including perforin+granzyme B (GZMB)+, interferon (IFN)-γ+CD107a+ and tumor necrosis factor (TNF)-α+CD107a+ NK cells, were significantly increased in primary NK cells co-cultured with 8d-pretreated HCC cells compared with those co-cultured with control or TP238-pretreated HCC cells (Figures 4G–4I). Here, PRF1 is linked to hepatocellular carcinoma.