As depicted in Figures 4C–4F, when human primary NK cells were co-cultured with 8d-pretreated Huh7 cells, the levels of activating receptors, including NCRs (NKp30, NKp46), NKG2D, and CD69 were significantly elevated compared with those co-cultured with control or TP238-pretreated HCC cells. This evidence concerns the gene KLRK1 and hepatocellular carcinoma.