We subsequently analyzed 45 GC tissues and divided them into HCMV-positive and HCMV-negative groups, and the results revealed that PD-L1 expression levels in HCMV-positive gastric cancer tissues (Mean ± SEM: 0.893 ± 0.186, N = 12) were significantly higher compared to HCMV-negative tissues (Mean ± SEM: 0.621 ± 0.106 N = 33). Here, CD274 is linked to gastric cancer.