To clarify the abnormal expression of ZCCHC4 in esophageal cancer, the ZCCHC4 mRNA level of ESCC tumors and adjacent non-tumor tissues was detected by qRT-PCR (Fig. 1B), which proved that tumor tissues were significantly higher than the control group, and this result is consistent with the results in the TCGA database (Fig. 1C). Here, ZCCHC4 is linked to esophageal squamous cell carcinoma.