To validate whether ZCCHC4 knockdown inhibits c-myc through highly reactive oxygen species (ROS), thereby modulating the proliferation of esophageal cancer cells, N-acetylcysteine (NAC) effectively reduced the elevated levels of ROS that were observed following the knockdown of ZCCHC4 (Fig. 5D). The gene discussed is MYC; the disease is esophageal cancer.