ZCCHC4 and esophageal squamous cell carcinoma: Next, we employed flow cytometry to investigate the effects of ZCCHC4 overexpression on cell cycle progression and apoptosis in ESCC cells, compared with the control group, the G0/G1 phase of the cell cycle was reduced (Fig. 6F), whereas the S phase was increased, and apoptosis was decreased (Fig. 6G) in the ZCCHC4-overexpressing group.