ZCCHC4 and esophageal cancer: To clarify the abnormal expression of ZCCHC4 in esophageal cancer, the ZCCHC4 mRNA level of ESCC tumors and adjacent non-tumor tissues was detected by qRT-PCR (Fig. 1B), which proved that tumor tissues were significantly higher than the control group, and this result is consistent with the results in the TCGA database (Fig. 1C).