To define the effects of SEC24 in STING signaling in an isogenic model, we deleted SEC24C using CRISPR-Cas9 in a cutaneous melanoma cell line (WM266.4) (Figure 4A) and a human leukemia monocytic cell line (THP-1) (Figure S4A) and evaluated the transcriptional induction of interferon target genes following stimulation with STING agonists. Here, SEC24B is linked to melanoma.