The clustering of PCA results showed that except for genes directly linked to cholesterol nucleation (MUC1 and MUC5ac), the expression patterns of other genes involved in cholesterol gallstone formation and NAFLD progression were significantly different between miceC70‐KO on the WD with dietary GlyMCA and TβMCA (Figure S8A–D, Supporting Information). Here, MUC1 is linked to metabolic dysfunction-associated steatotic liver disease.