To study the effects of nestin+ cell depletion in the two AML models, Nes-creERT2;R26lacZbpAfloxDTA mice (abbreviated as Nes-creERT2;iDTA) were intercrossed with iMLL-AF9 mice (iMLL-AF9;Nes-creERT2;iDTA) or transplanted with BM cells from FLT3-ITD;NPMc mice (FLT3-ITD;NPMc; iDTA), together with control helper cells. The gene discussed is FLT3; the disease is acute myeloid leukemia.