Subsequent clustering analysis of T cells, B cells, myeloid, and thyroid follicular cells revealed that the abundance rates of the CD8+T_CCL4L2, B_MEF2B_BCL6, Mac_APOE, Mac_IL1B, and TFC_PAX8_NKX2‐1 subgroups were elevated in thyroid tissues of HT patients. The gene discussed is NKX2-1; the disease is hematocrit.