Studies demonstrate that RBP Aly/REF export factor (ALYREF) is involved in tumor cell proliferation and metastasis.19–21 Notably, ALYREF has been indicated to be upregulated in NSCLC and exert tumorigenic functions in this cancer.22 Here, we used ENCORI database to predict the upstream molecules of TTYH3 and found that ALYREF was a likely target of LUCAT1 and it could target TTYH3. The gene discussed is TTYH3; the disease is cancer.