The analytes we found to be elevated in CF were associated with traditional inflammation as observed previously, including TNF-α,14 IL-1β,14 IL-8,15, 19 IL-6,14, 1620 MIP-1α,15 MIG15 and MCP-115 16 21; however, we also showed strong enrichment for type 2 inflammatory responses, including elevation of IL-4, IL-13, Eotaxin and FGF- 210 in both BAL and peripheral blood, highlighting that these type 2 cytokines also play a key role in the establishment of inflammation in early CF. This evidence concerns the gene CCL3 and cystic fibrosis.