DNMT1 and cancer: 363,364 The catalytic domain of DNMT3a was fused to its activating partnerDNMT3L to render it constitutively active, and the active site cysteinewas photocaged.365 Colon cancer cells withnative DNMT1 and DNMT3b genes knocked out were cotransfected with E. coli machinery DMNBSRS and the DNMT-C710TAG fusion construct.Successful amber suppression with DMNBC was read outthrough anti-HA immunostaining and flow cytometry.