CASP3 and infection: However, if 2B* is acting to induce caspase-3-dependent (GSDME-mediated) pyroptosis (which WT EMCV is known to cause [34]), then 2B*KO EMCV-infected cells would have less cleaved caspase-3 than WT EMCV-infected cells, at timepoints where lysis is reduced in 2B*KO EMCV infection.