In addition, in this HFDHFr model of MASH and fibrosis, fibrosis was predominant in zone 2 (perisinusoidal region) of the liver lobule, and Mtarc1 knockdown resulted in significantly lower zone 2 fibrosis (Figure 7C) and decreased fibrosis-steatosis co-localization for (Figure 7D). This evidence concerns the gene MTARC1 and steatosis.