Compared to the HEK-293 WT cells, the mRNA expression of ZIKV E in HEK-293 EXT1-KO cells was significantly decreased from 24 hours post-infection (hpi) to the observation endpoint, 72 hpi, and no significant difference was observed at the early time points (6, and 12 hpi) (Figure 1b). The gene discussed is EXT1; the disease is infection.