TNF and infection: Sendai 16,226 infection increased the transcript levels of the intracellular cytokines IL-6 and IL-8 (with a relative mRNA level of 7 and 14 to cells not be infected which were obtained by the 2-ΔΔCt calculation method using GAPDH as an internal reference gene) but not as well on those of INF-γ and TNF-α (only 1.9 and 2.4 relative to cells not be infected).