NANOS1 and breast cancer: Among these, NANOS1, a less studied and the only downregulated gene, was chosen for further analysis. The prognostic value of NANOS1 protein expression in breast cancer was evaluated using the online tool available at www.proteinatlas.org, and the results demonstrated that lower expression of NANOS1 was associated with better prognosis (p < 0.001) (Figure 2B).