According to the heatmap, the Tp53 gene mutation frequency in CRC tissue samples was the highest at 65.22%, followed by the PIK3CA (39.13%), KRAS (30.43%), BRAF (17.39%), APC (26.09%) and EGFR (34.78%) genes (Figure 6A). The gene discussed is PIK3CA; the disease is colorectal carcinoma.