MUC2 and cancer: While both in 2D and 3D, we could observe a decrease in cancer cells with every increase in the amount of E/A PBMCs plated on the cancer cells, but no significant difference could be observed between CTRL and MUC2 K.O. in both cell lines (Supplementary Figure S14A–F), though we could identify a higher sensitivity of HT-29 cells compared to LS-174T in both CTRL and MUC2 K.O. to co-culture with 500 E/A PBMCs in 2D and co-culture with 1,000 E/A PBMCs in 3D (Figure 4A).