In conclusion, we generated a unique zebrafish animal model of TNNI3K splicing mutation-based cardiomyopathy and CCD and identified the focal adhesion pathway and Mypt1/Mlc2/Yap1/Nfatc1 axis as the downstream phosphorylation targets of the Tnni3k protein. This evidence concerns the gene PPP1R12A and cardiomyopathy.