To address this research gap, in this study a mouse model of LPS-induced ALI, murine lung epithelial cell-12 (MLE-12) cells, and macrophage models were used to study the therapeutic mechanisms of YNJ on ALI, with particular focus on the potential regulation of the NF-κB/NLRP3 pathway and pyroptosis. The gene discussed is NFKB1; the disease is acute respiratory distress syndrome.