Ten lncRNAs were selected for validation by qRT-PCR, including five upregulated lncRNAs (RMRP, RN7SL2, RPPH1, CTD-2184D3.5, and linc-ROR) and five downregulated lncRNAs (ZNRF3-IT1, RP11-431K24.1, RP11-32B5.7, RN7SL4P, and MALAT1) in serum EVs from patients with GC compared with healthy controls. This evidence concerns the gene RPPH1 and gastric cancer.