We also stably silenced NSUN2 in the HCC cell lines Hep3B and Huh7 via lentivirus-mediated short hairpin RNAs (NSUN2-sh1, NSUN2-sh2 and NSUN2-sh3) (Supplementary Fig. 2B, C). The gene discussed is NSUN2; the disease is hepatocellular carcinoma.