To determine the role of mRNA m5C modification in HCC, we evaluated the expression levels of two major mRNA m5C methyltransferases, NSUN2 and NSUN6 (the only two known RNA m5C methyltransferases that methylate mRNA [7, 10]), in 40-paired HCC and ANL tissues via RT-qPCR (Cohort 1). Here, NSUN6 is linked to hepatocellular carcinoma.