Furthermore, we detected the expression of these seven mRNAs in HCC cell lines and found that only PKM2 was upregulated after NSUN2 was overexpressed in both HepG2 and SNU387, cells but downregulated after NSUN2 was silenced in both Hep3B and Huh7 cells (Fig. 4D, E). The gene discussed is PKM; the disease is hepatocellular carcinoma.