To confirm whether the effect of TRPM3 modulators on cyst formation was cAMP-dependent, we cultured E12.5 wild-type mouse kidneys immediately after dissection in KCM supplemented with TRPM3 modulators (isosakuranetin and nifedipine) along with varying concentrations of IBMX (3-isobutyl-1-methylxanthine). The gene discussed is TRPM3; the disease is cyst.