In order to evaluate whether this interaction would be mediated by the ERα RNA binding domain (RBD) identified by Xu et al. [4], the same constructs generated and kindly provided by these authors were used here by applying RIP coupled to RT-qPCR in a system composed of Hs-578T (ERα-negative BC cell line) stably expressing full-length-3xFlag-ESR1 (Flag-ERα) with or without mutation in its RBD. Here, ESR1 is linked to breast cancer.