On day 8th of embryo development, to achieve PVT1 silencing MCF-7 cells were in-plate transfected for 6 h, to allow the maximal effect of liposomes, as detailed in methods section, then they were detached and 5 ×106 cells were inoculated into the CAMs; the tumor formation was then evaluated after 4 days. The gene discussed is PVT1; the disease is neoplasm.