HDAC1 and breast cancer: To confirm their divergent expressions and function under anaerobic conditions of BC cells, we detected the H3k18la and HDACs (HDAC1/2/3) levels in MB-231 cells by qPCR and WB assays, showing that the level of H3K18la was significantly elevated in the ROT group compared to the DCA group and the control group (Fig. 2A, B).