Next, expression of H3K18la and HDACs were measured by IF staining and qPCR assay in breast cancer tissues and para-cancerous tissues of patients (n = 24; Fig. 2E; Supplementary Fig. 1D), exhibiting that H3K18la expression was higher in BC tissues than that in BC para-cancerous tissues, while HDAC2 and HDAC3 decreased in BC para-cancerous tissues. Here, HDAC3 is linked to breast cancer.