To further elucidate the role of GFI1 in regulating pulmonary ACOD1 expression in the context of obesity, we specifically knocked down GFI1 expression in the lungs of HFD mice via airway administration of adeno‐associated virus (shGFI1, 5 × 1012 vg ml−1; 100 μl mouse−1, 5 × 1011 vg mouse−1) (Figure S8C, Supporting Information). The gene discussed is GFI1; the disease is obesity due to melanocortin 4 receptor deficiency.