We explored the effects of P4HA2, HIF-1α, and HILPDA in colon cancer cell lines, the results suggested that overexpression of P4HA2, HIF-1α, and HILPDA promoted lipid droplet accumulation in colon cancer cells by reducing ATGL enzyme activity (Figs. S6A–E) and promoted the proliferation and migration of colon cancer cells (Figs. S6F–J). This evidence concerns the gene PNPLA2 and malignant colon neoplasm.