Further, we confirmed this phenomenon in glioma cell lines, that the acquisition of R132C or R132H variants of IDH1 resulted in remarkably increased formation of γH2A.X puncta in cell nuclei and modification of γH2A.X level when exposed to genotoxic agents such as temozolomide (TMZ) or carmustine (BCNU) (Fig. 1a, b and Supplementary Fig. 2a). Here, IDH1 is linked to central nervous system cancer.