KEGG pathway enrichment analyses further unveiled the involvement of SEPN1 in various pathways, such as Th17 cell differentiation, Th1 and Th2 cell differentiation, neutrophil extracellular trap formation, chemokine signaling pathway, B cell receptor signaling pathway, transcriptional misregulation in cancer, proteoglycans in cancer, cell cycle, glioma, TNF signaling pathway, and apoptosis (Fig 4E–4H and S16-S19 Tables in S1 File). The gene discussed is TNF; the disease is central nervous system cancer.