Our previous evidence showed that the deficiency in the expression of complex branched N-glycans in Mgat5 knockout mice (Mgat5-/-) resulted in an early onset colitis in DSS-induced model, with mice developing severe forms of intestinal inflammation (Supplementary Figure S1A).20 However, whether this deficiency on mucosa glycome may impact microbiota composition associated with colitis susceptibility remains unknown. This evidence concerns the gene MGAT5 and inflammatory response.