In this study, we demonstrate the use of two probes: 2-NBDG and TMRE with both a standard fluorescence microscope (ZOETM, Bio-Rad) and a flow cytometry device (BD Symphony A3), to report the changes in metabolism between radioresistant (rSCC-61) and radiosensitive (SCC-61) HNSCC cells under radiation stresses with or without HIF-1α inhibition.36 Our studies found that rSCC-61 cells have increased baseline glucose uptake and decreased baseline MMP compared with SCC-61 cells. The gene discussed is HIF1A; the disease is head and neck squamous cell carcinoma.