To further reveal the role of radiation-induced HIF-1α expression in the HNSCC radioresistance development, we further conducted western blotting experiments on rSCC-61 and SCC-61 cells with various treatments, including no treatment (control), 4 Gy radiation-only treatment, HIF-1α inhibition only, and 4 Gy radiation treatment plus HIF-1α inhibition. Here, HIF1A is linked to head and neck squamous cell carcinoma.