Furthermore, we pinpointed 9 key regulatory genes (TUBA1A, ICAM4, SCEL, TMPRSS11A, TMPRSS11B, IGFBP2, CLC, NFAM1, and F13A1) in BALF of asthma by integrating scRNA-seq and bulk RNA-seq data. This evidence concerns the gene IGFBP2 and asthma.