Diagnosis of HCC is further supported by immunohistochemistry for markers glypican-3 (GPC3: NCT05003895, NCT05103631), epithelial cell adhesion molecule (NCT05028933, NCT03013712), MET (NCT01755767), mucin 1 (NCT02587689), MHC1 (NCT05195294), and TERT (NCT05595473), are being incorporated into these clinical trials. This evidence concerns the gene GPC3 and hepatocellular carcinoma.