Compared to controls and AsymC9, microtubule‐associated protein tau (MAPT), phosphorylated tau 181 (pTau181), phosphorylated tau 217 (pTau217), phosphorylated tau 231 (pTau231), and phosphorylated TDP‐43 (pTDP‐43) were elevated in ALS. NEFL levels positively correlated with pTau181, pTau217, pTau231, and pTDP‐43. MAPT and pTDP‐43 were also correlated with pTau181, pTau217 and pTau231. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.