To validate the possibility of Trx1 interacting with the native fusion process, we conducted a straightforward pseudovirus infection assay of JRFL Env, BaL.01 Env, HXB2 Env, and vesicular stomatitis virus (VSV) pseudovirus with HOS CD4+ CCR5+ target cells supplemented with serial dilutions of Trx1. This evidence concerns the gene TXN and infection.