MNX1 is a homeobox domain‐containing transcription factor, which has been reported to be located in the nucleus[55] and transcriptionally regulate gene expression in human cancer cells.[30, 33, 34] In our study, IHC staining showed that MNX1 was indeed predominantly located in the nucleus in para‐tumor tissues, but in ESCC tissues, MNX1 was localized in both cytoplasm and nucleus, and cytoplasmic MNX1 was positively associated with PD‐L1 expression. This evidence concerns the gene CD274 and esophageal squamous cell carcinoma.