Given that plasma membrane PD‐L1 on cancer cells interacts with PD‐1 on T cells to evade anti‐tumor immunity, we then employed flow cytometry to analyze the levels of PD‐L1 on plasma membrane, and observed that the membrane PD‐L1 was decreased upon MNX1 depletion in KYSE150, MCF7, and 4T1 cells (Figure 2k). The gene discussed is PDCD1; the disease is cancer.