To verify the high MNX1 expression in ESCC, we performed RT‐qPCR and western blotting assays, and observed that the expression of MNX1 in most ESCC cell lines and tissues was higher than that in immortalized esophageal epithelium cell line NE2 and paired para‐tumor tissues, respectively (Figure 6c–e). The gene discussed is MNX1; the disease is esophageal squamous cell carcinoma.