For the females, as the quartiles of SPISE increased, the duration of diabetes, HDL-c showed a gradual increased, while the age, BMI, VFA, SFA, SBP, DBP, TC, LDL-c, TG, TG/HDL-c, FPG, ALT, AST, GGT, UA, Hb, FINS, HOMA-IR and the incidence of NAFLD exhibited a gradual decreased (all P < 0.05). This evidence concerns the gene GSTM1 and metabolic dysfunction-associated steatotic liver disease.