To further verify the distinct immune compositions in hot and cold tumors and their implications on patient survival, we conducted an mIHC analysis using a tissue array of 71 patients with PAAD to identify the expression levels of CD8 (a marker of CD8 T cells), CD163 (a marker of M2 macrophages), and PANCK (a marker of cancer cells) (Figures 2A–D). The gene discussed is CD8A; the disease is cancer.