Premature infants have low arginine and intake, Kam and colleagues discovered that the expression of arginine synthesizing enzymes, including ALDH18A1, ASL, ASS1, CPS1, GLS, OAT, OTC and PRODH were significantly decreased in the in NEC tissues (Leung et al., 2016). This evidence concerns the gene ALDH18A1 and necrotizing enterocolitis.