Similarly, TGF-β1 stimulation did not impact the modal size, but increased significantly the numbers of EV+ TGF−β1 released by A549 (LUAD), H1299 (non-small cell lung carcinoma), MCF7 (luminal-A BRCA), MCF10A (non-tumorigenic breast epithelial) and BNF3 (normal breast fibroblast) cells (Supplementary Fig. S1C), suggesting a general stimulatory effect of TGF-β on EV secretion by different tumor and non-tumorigenic cells. The gene discussed is TGFB1; the disease is neoplasm.