EA treatment significantly increased the phosphorylation of FAK (Fig. 6E), TGF-βR1 (Fig. 6F), and Smad2 (Fig. 6G) in the PCOS + EA + Scramble group, but these effects were diminished in the PCOS + EA + Itgb1 knockdown group. The gene discussed is PTK2; the disease is polycystic ovary syndrome.