MKI67 and neoplasm: Significantly higher tumor weight and growth rate were observed in the D-2HG group as compared to mice treated with DMSO (Fig. 2E-G). The immunohistochemistry assay was performed to evaluate the proliferative and metastatic levels in the xenograft tumors. The tumors treated with D-2HG exhibited increased Marker of proliferation Ki-67 (MKI67) and vimentin (VIM) activity while a decreased ECAD signal compared to the DMSO group (Fig. 2H).