FOS and Anxiety: Specifically, within the amygdala, a critical component of the neural circuitry underlying anxiety-related behavior, we observed a significant increase in the number of c-Fos-positive cells within the basolateral amygdala (BLA), but not the central amygdala (CeA), of GF mice compared to SPF mice (SPF: 182.8 ± 24.62 n/mm2; GF: 343.8 ± 7.62 n/mm2; Student’s t test <0.0001) (Fig. 1E,I).