As mentioned above, while we did not find a correlation between the location of dysfunctional subsets of CD8 + T cells (Tim3 + CD8+ and Tim3-CD8) and G9 expression levels in tumor cells (see Fig. 1H), the GzB-expressing CTLs likely to engage more physically with neighboring tumor cells in G9-low compared to G9-high tumor regions, indicating cell-associated G9 exerts inhibitory effect on the cytotoxicity potential of CTLs. This evidence concerns the gene CD8A and neoplasm.