Still, the CCL4 control group recorded marked hepatic degenerative changes indicating cytoplasmic eosinophilia and nuclear pyknosis and vacuolation of hepatocytes associated with perivascular fibroblastic activity marked hepatic necrotic changes associated with obvious periportal and per lobular hepatic fibrosis (Fig. 6B). This evidence concerns the gene CCL4 and Increased total eosinophil count.