Western blotting showed that the exosome markers Hsp90 and CD81 did not significantly differ among the control exosomes, Exo-siNC, and Exo-siTMEM45A, were scarcely present in primary tumor cells, while α-Tubulin was expressed in primary cells but at lower levels in exosomes (Fig. 5E). This evidence concerns the gene CD81 and neoplasm.